Spectra of spontaneous mutations at the hprt locus in colorectal carcinoma cell lines defective in mismatch repair
نویسندگان
چکیده
منابع مشابه
Spectra of spontaneous mutations at the hprt locus in colorectal carcinoma cell lines defective in mismatch repair.
Spectra of spontaneous mutations at the hypoxanthine-guanine phosphoribosyltransferase (hprt) locus in colon carcinoma cell lines HCT116 and HCT-15 deficient in mismatch repair and displaying mutator phenotypes were determined. HCT116 and HCT-15 cells, respectively, harbour a mutation in the mismatch repair gene hMLH1 and GTBP. The mutation frequency at the hprt locus in both cell lines was ele...
متن کاملDefective mismatch repair in extracts of colorectal and endometrial cancer cell lines exhibiting microsatellite instability.
A replication error (RER+) phenotype, characterized by somatic instability in simple repeated sequences, is associated with several types of cancer. To determine if a defect in DNA replication fidelity or repair of replication errors might explain this instability, we compared both processes in cell-free extracts from RER+ endometrial and colorectal cancer cell lines to RER- cell lines. SV40 or...
متن کاملNeocarzinostatin-induced mutations at the hprt locus in exponentially growing CHO cells, compared with spontaneous mutations.
Spontaneous mutations and neocarzinostatin-induced mutations were investigated in the hypoxanthine-guanine phosphoribosyltransferase (hprt) gene in exponentially growing Chinese hamster ovary cells. Neocarzinostatin (NCS) treatment caused an 4.5-fold increase in mutation frequency. Analysis by multiplex polymerase chain reaction and sequencing of hprt cDNA revealed that spontaneous mutations in...
متن کاملMutation Rates, Spectra, and Genome-Wide Distribution of Spontaneous Mutations in Mismatch Repair Deficient Yeast
DNA mismatch repair is a highly conserved DNA repair pathway. In humans, germline mutations in hMSH2 or hMLH1, key components of mismatch repair, have been associated with Lynch syndrome, a leading cause of inherited cancer mortality. Current estimates of the mutation rate and the mutational spectra in mismatch repair defective cells are primarily limited to a small number of individual reporte...
متن کاملRepair-defective Human Tumor Cell Lines Expression of hMLH1 in Sporadic Colon Tumors and Mismatch
Somatic mutations in DNA mismatch repair genes have been observed in sporadic tumors as well as ceHlines and xenografts derived from such tumors implicating genetic defects of mismatch repair genes in the devel opment of such tumors. However, the proportion of sporadic tumors in which mismatch repair genes have been inactivated has not been deter mined accurately. We have analyzed 66 sporadic c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Carcinogenesis
سال: 1997
ISSN: 1460-2180
DOI: 10.1093/carcin/18.6.1127